Well a successful trip to Zurich and I am pleased to say that we managed to get a very respectable SoFAST-HMQC on FIVE micrograms of 15N-labelled S. coelicolor ACP in a 1.7mm tube dissolved in 35 micro litres of phosphate buffer. The 1H-15N HSQC is shown below. A big thank-you to Frank Schumann and Rainer Kummerle at Bruker for their assistance. I should add that this data was collected in under 20 minutes!ACP