Biosynthesis

Recent Review: Polyketide beta-branching: diversity, mechanism and selectivity

mattcrump Uncategorized , ,

The Crump group is pleased to announce the publication of a recent review within Natural Product Reports. This review summarises the diversity of beta-branch incorporation within polyketide synthases and the mechanistic details of each catalytic step. The overall transformation is catalysed by a multi-protein 3-hydroxy-3-methylglutaryl synthase (HMGS) cassette and is reminiscent of the mevalonate pathway in terpene biosynthesis. The application of HMGS in data mining, additional beta-branching mechanisms and current knowledge of the role of beta-branches in this important class of biologically active natural products is also discussed.

Recent Paper: A Priming Cassette Generates Hydroxylated Acyl Starter units in Mupirocin and Thiomarinol Biosynthesis.

mattcrump Recent Publications , , ,

The Crump group is pleased to announce the publication of recent work in ACS Chemical Biology Journal. This work was completed as a collaboration between the Departments of Chemistry and Biochemistry at the University of Bristol, showing the need for frequent collaboration in the field of biosynthesis of natural products.

This work utilises a plethora of laboratory techniques to fully characterise the formation of a 3-carbon alcohol intermediate in the biosynthesis of the polyketide antibiotic mupirocin and homologous 4-carbon alcohol intermediate in the biosynthesis of thiomarinol.

This is exciting work and provides a solid contribution to the biosynthetic community, well done to all involed in the publication of this work.

A link to the paper can be found at the following link: https://doi.org/10.1021/acschembio.9b00969